Methane Production/Oxidation Potential and Methanogenic Archaeal Diversity in Two Paddy Soils of Japan

Ankit Singla, Rosnaeni Sakata, Syunsuke Hanazawa, Kazuyuki Inubushi

Abstract


Soil characteristics regulate various belowground microbial processes and consequently affect the structure and function of microbial communities due to change in soil type which in turn influences the CH4 production/oxidation potential of soils. Thus, two different soil types (Andosol and Regosol) were studied to assess their CH4 production/oxidation potential and also the methanogenic archaeal diversity in Japanese paddy soils. Andosol produced significantly higher concentration of CH4 and CO2 under waterlogged incubation. It also consumed CH4 quicker than Regosol soil and also produced significantly higher concentration of CO2 under aerobic incubation. The cumulative CH4 production was found to be significantly correlated with soil’s physico-chemical properties. Denaturing Gradient Gel Electrophoresis showed differences in methanogenic archaeal banding pattern in both soils. The present study suggested that CH4 production/oxidation potential of different soils depends on physico-chemical properties and microbial communities.

Keywords


Methane Oxidation; Carbon Dioxide Production; Soil Characteristics; DGGE· Methanogens; Andosol; Regosol

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