Molecular Characterization, Statistical analysis of protease and its application

Sreecha Chandran Nair, A Gangaprasad, A Jayakumaran Nair

Abstract


Proteolytic enzymes are currently attracting enormous attention because of their biotechnological potential. Currently, bacterial proteases are in great demand because of potential industrial applications that are found in soil or mud. This research was conducted to find the proteolytic bacteria isolation from PKC (Palm Kernel Cake) sample collected from Oil Palm India Limited, Kollam, Kerala and it was screened for a novel protease producing bacteria in Skim milk agar medium. Among the isolates, the S1 isolate exhibited high proteolytic activity and was selected as a novel protease-producing bacteria, which was identified by morphological and biochemical characteristics as Pseudomonas aeruginosa. The optimization study was carried out for pH, carbon, substrate, surfactants, nitrogen, metal ions, and inoculum size for determining the maximum production of protease. The results are stated thereby.

Keywords


Protease, Pseudomonas aeruginosa, Statistical analysis

References


Bezerra, M. A., Santelli, R. E., Oliveira, E. P., Villar, L. S., & Escaleira, L. A. (2008). Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta, 76(5), 965-977.

Clarke, L., Kyle, P., Wise, M., Calvin, K., Edmonds, J., Kim, S., ... & Smith, S. (2008). CO2 emissions mitigation and technological advance: an updated analysis of advanced technology scenarios. PNNL Report Pacific Northwest National Laboratory, Richmond.

Kalisz, H. M. (1988). Microbial proteinases. Enzyme studies, 1-65.

Kumar, C. G., & Takagi, H. (1999). Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnology advances, 17(7), 561-594.

Munawar, A., Trusch, M., Georgieva, D., Hildebrand, D., Kwiatkowski, M., Behnken, H., ... & Betzel, C. (2014). Elapid snake venom analyses show the specificity of the peptide composition at the level of genera Naja and Notechis. Toxins, 6(3), 850-868.

Pillai, P., & Archana, G. (2008). Hide depilation and feather disintegration studies with keratinolytic serine protease from a novel Bacillus subtilis isolate. Applied Microbiology and Biotechnology, 78(4), 643-650.

Rosselló‐Mora, R. A., Lalucat, J., Dott, W., & Kämpfer, P. (1994). Biochemical and chemotaxonomic characterization of Pseudomonas stutzeri genomovars. Journal of applied bacteriology, 76(3), 226-233.


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