Dhanalakshmi J, Jeevan P


Algal biofuel or algal oil is an alternative to liquid fossil fuels that uses algae as the source of energy and they are considered as a replacement for biofuel sources such as corn and sugarcane. This study presents the isolation of algal strain from fresh water ponds in Pudukkottai District. The algal strain was confirmed as Microseira wollei after PCR amplification and sequencing of the 16s rRNA gene of the DNA isolated from the species. The structure of the strain was observed using a Trinocular microscope. The phylogenetic tree analysis was carried out to find the evolutionary relationship of the organism. Biodiesel was produced from Microseira wollei by lipid extraction and allowed for trans-esterification. The biodiesel production of was Microseira wollei was confirmed by GC-MS analysis which showed high quantity of methyl ester compounds.

Keywords: Microseira  woelli, biodiesel, 16s rRNA gene, trans-esterification,  GC-MS


Biofuel production


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