Biomass Production, Chlorophyll Content and Morphorogical Parameters Are Affected by Sulfur Deficiency in Eruca sativa L.

Mariame Houhou, Khalid Amrani Joutei, Said Louhalia


Eruca sativa is one of the most cultivated brassica belonging to medicinal and aromatic species, The effect of sulphur deprivation on the growth and metabolism of Eruca sativa was investigated by measuring dry weights of the plant, chlorophyll content, total sulphur, leaf area, root/shoot ratio (R/S), Leaf Area Ratio (LAR), Specific Leaf Area (SLA) and leaf weight ratio (LWR). Significant decrease was observed in total dry weight of the overall plant. Both total sulphur and sulphate concentrations were markedly reduced in response to sulphur deficiency, significant decrease was recorded in chlorophyll concentration. Decreases were also observed in Leaf area and R/S ratio, while, a significant increase was recorded in LAR and SLA.


Chlorophyll; Eruca sativa;, Leaf area; SLA; LAR; Sulfur Deficiency


Abdallah, M.; Dubousset, L.; Meuriot, M.; Etienne, P.; Avice J.C. and Ourry, A.. 2010. Effect of mineral sulfur availability on nitrogen and sulfur uptake and remobilization during the vegetative growth of Brassica napus L. Journal of Experi-mental Botany 61(10): 2635-2646.

Akiko, M.N.; Yumiko, N.; Akiko, W.T.; Eri, I.; Tomoyuki, Y. and Hideki, T. 2005. Identification of a novel cis-acting element conferring sulfur deficiency response in Arabidopsis roots. Plant Journal 42: 305-314.

Arnon, D.I. 1949. Copper enzymes isolated chloroplasts, poly-phenoloxidase in Beta vulgaris. Plant Physiology 24: 1-15.

Astolfi, S. and Zuchi, S. 2013. Adequate S supply protects barley plants from adverse effects of salinity stress by increasing thiol contents. Acta Physiologiae Plantarum 35: 175-181.

Bardsley, C.E. and Lancaster, J.D. 1960. Determination of reserves sulphur and soluble sulfate in soil. Proceedings Soil Science Society of America 24: 265-268.

Bell, C.I.; Clarkson, D.T and John Cram, W.1995. Partitioning and redistribution of sulphur during S-stress in Macroptilium atropurpureum cv. Siratro. Journal of Experimental Botany 46: 73-81.

Blake-Kalff, M.M.A.; Harrison, K.R.; Hawkesford, M.J.; Zhao, F.J. and McGrath, S.P. 1998. Distribution of sulfur within oilseed rape leaves in response to sulfur deficiency during vegetative growth. Plant Physiology 118: 1337 - 1344.

Bennett, R.N.; Mellon, F.A.; Botting, N.P.; Eagles, J.; Rosa, E.A.S. and Williamson, G. 2002. Identification of the major gluco-sinolate (4-mercaptobutyl glucosinolate) in leaves of Eruca sativa L. (salad rocket). Phytochemistry 61: 25-30.

Burke, J.J.; Holloway, P. and Dalling, M.J. 1966. The effect of sulfur deficiency on the organization and photosynthetic capability of wheat leaves. Journal of Plant Physiology 125: 371-375.

Casieri, L.; Gallardo, K. and Wipf, D. 2012. Transcriptional response of Medicago truncatula sulfate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress. Planta 235: 1431-1447.

Clarkson, D.T.; Smith, F.W. and Vanden, B.P.J. 1983. Regulation of sulfate transport in a tropical legume, Macroptilium atro-purpureum, cv. Sirato. Journal of Experimental Botany 34: 1463-1483.

Davidian, J.C. and Kopriva, S. 2010. Regulation of sulfate uptake and assimilation-the same or not the same? Molecular Plan 3: 314-325.

Droux, M. 2004. Sulfur assimilation and the role of sulfur in plant metabolism: a survey. Photosynthesis Research 79 : 331-348.

Erice, G.; Louahlia, S.; Irigoyen, J.J.; Sanchez-Diaz, M. and Avice, J.C. 2010. Biomass partitioning, morphology and water status of four alfalfa genotypes submitted to progressive drought and subsequent recovery. Journal of Plant Physiology 167: 114-120.

Gao, Y.; Li, X.; Tiana, Q.Y.; Wanga B.L. and Zhanga, W.H. 2015. Sulfur deficiency had different effects on Medicago truncatula ecotypes A17 and R108 in terms of growth, root morphology and nutrient contents. Journal of Plant Nutrution 39: 301-314.

Garnier, E.; Salager, J.L.; Laurent, G. and Sonie, L. 1999. Relation-ships between photosynthesis, nitrogen and leaf structure in 14 grass species and their dependence on the basis of expression. New Phytologist 143: 119-129.

Grant, C. and Hawkesford, M.J. 2015. Sulfur. Pages 261-304, In : Barker, A.V. and D.J. Pilbeam, D.J. (Editors). Handbook of Plant Nutrition, 2nd Edition. CRC Press, Boca Raton, FL.

Guendouz, A.; Semcheddine, N.; Moumeni, L. and Hafsi, M. 2016. The effect of supplementary irrigation on leaf area, specific leaf weight, grain yield and water use efficiency in durum wheat (Triticum durum Desf.) cultivars. Journal of Plant Breeding and Crop Science 2: 82-89.

Haneklaus, S.; Bloem, E.; Schnug, E.; De Kok, L.J. and Stulen, I. 2007. Sulfur. Pages 183-238, In: Barker, A.V. and Pilbeam, D.J. (Editors). Handbook of Plant Nutrition. CRC Press, Boca Raton, FL.

Hawkesford, M.J. 2000. Plant responses to sulphur deficiency and the genetic manipulation of sulphate transporters to improve S-utilization efficiency. Journal of Experimental Botany 51: 131-138.

Hermans, C.; Hammond, J.P.; White P.J. and Verbruggen, N. 2006. How do plants respond to nutrient shortage by biomass allocation? Trends in Plant Sciences11: 610 - 617.

Hiscox, J. and Israelstam, D.G.F. 1949. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332-1334.

Hoagland, D.R. and Arnon, D.I. 1950. The Water-Culture Method for Growing Plants Without Soil. 2nd edition. California Agri-cultural Experiment Station Circular 347. College of Agriculture, University of California, Berkeley, CA. 32 pages.

Hoefgen, R. and Nikiforova, V.J. 2008. Metabolomics integrated with transcriptomics: assessing systems response to sulfur-deficiency stress. Plant Physiology 132: 190-198.

Juszczuk, I.M. and Ostaszewska, M. 2011. Respiratory activity, energy and redox status in sulphur-deficient bean plants. Environmental and Experimental Botany 74: 245-254.

Kassem, A.S.; Mohammed H.F.A. and EL-Sayed, S.A.A. 2015. Influence of sulfur deprivation on biomass allocation, mineral composition and fruit quality of tomato plants. Middle East Journal of Agriculture Research 4: 42-48.

Klimont, Z.; Smith, S.J. and Cofala, J. 2013. The last decade of global anthropogenic sulfur dioxide: 2000-2011 emissions. Environmental Research Letters 8: 014003.

Kutz, A.; Muller, A.; Hennig, P.; Kaiser, W.M.; Piotrowski, M. and Weiler, E.W. 2002. A role for nitrilase 3 in the regulation of root morphology in sulphur-starving Arabidopsis thaliana. Plant Journal 30: 95-106.

Leustek, T.; Martin, M.N.; Bick, J.A. and Davies, J.P. 2000. Pathways and regulation of sulfur metabolism revealed through molecular and genetic studies. Annual Review of Plant Physiology and Plant Molecular Biology 51: 141-165.

Leustek, T. and Saito K. 1999. Sulfate transport and assimilation in plants. Plant Physiology 120: 637-643.

Lopez-Bucio, J.; Cruz-Ramırez, A. and Herrera-Estrella, L. 2003. The role of nutrient availability in regulating root architecture. Current Opinion in Plant Biology 6: 280-287.

Lunde, C.; Zygadlo, A.; Simonsen, H.T.; Nielsen, P.L.; Blennow, A. and Haldrup, A. 2008. Sulfur starvation in rice: the effect on photosynthesis, carbohydrate metabolism, and oxidative stress protective pathways. Physiologia Plantarum 134: 508-521.

Lynn, A.; Collins, A. Fuller, Z.; Hillman, K. and Ratcliffe, B. 2006. Cruciferous vegetables and colo-rectal cancer. Proceedings of the Nutrition Society, 65: 135-144.

Marshner, H. 2005. Mineral Nutrition of Higher Plants. Second edition. Elsevier Academic Press, London, UK.

Nielsen, S.L.; S. Enriquez, C.M. Duarte and Sand-Jensent, K. 1996. Scaling maximum growth rates across photosynthetic organism. Functional Ecology 10: 167-175.

Nikiforova V.J.; Daub, C.O.; Hesse, H.; Willmitze, L. and Hoefgen, R. 2005. Integrative gene-metabolite network with imple-mented causality deciphers informational fluxes of sulphur stress response. Journal of Experimental Botany 56: 1887-1896.

Nikiforova, V.; Freitag, J.; Kempa, S.; Adamik, M.; Hesse, H. and Hoefgen, R. 2003. Transcriptome analysis of sulfur depletion in Arabidopsis thaliana: interlacing of biosynthetic pathways provides response specificity. Plant Journal 33: 633-650.

Nikiforova,V.J.; Gakiere, B.; Kempa, S.; Adamik, M.; Willmitzer, L.; Hesse, H. and Hoefgen, R. 2004. Towards dissecting nutrient metabolism in plants: a systems biology case study on sulphur metabolism. Journal of Experimental Botany 55: 1861-1870.

Martinez-Sanchez, A.; Allende A.; Bennett, R.N.; Ferreres, F. and Gil, M.I. 2006. Microbial, nutritional and sensory quality of rocket leaves as affected by different sanitizers. Postharvest Biology and Technology 42: 86-97.

Pagani, A. and Echeverría, H.E. 2012. Influence of sulfur deficiency on chlorophyll-meter readings of corn leaves. Journal of Plant Nutrution and Soil Science 175: 604-613.

Prosser, I.M.; Purves, J.V.; Saker, L.R. and Clarkson, D.T. 2001. Rapid disruption of nitrogen metabolism and nitrate transport in spinach plants deprived of sulphate. Journal of Experimental botany 52: 113-121.

Rausch, T. and. Wachter, A. 2005. Sulfur metabolism: a versatile platform for launching defence operations. Trends in Plant Sciences 10: 503 - 509.

Ross, J.V. and Koppel, A. 2000. Estimation of leaf area and its vertical distribution during growth period. Agricultural and Forest Meteorology 101: 237-246.

Saito, K. 2004. Sulfur assimilatory metabolism, the long and smelling road. Plant Physiology 136: 2443 - 2450.

Scherer, H.W. 2001. Sulphur in crop Production. European Journal of Agronomy 14: 81-111.

Scherer, H.W.; Pacyna, S.; Spoth, K.R, and Schulz, M. 2008. Low levels of ferredoxin, ATP and leghemoglobin contribute to limited N2 fixation of peas (Pisum sativum L.) and alfalfa (Medicago sativa L.) under S deficiency conditions. Biology and Fertility of Soils 44: 909 - 916.

Syvertsen, J.P.; Lloyd, J.; Meconchie, C, Kriedbmann, P.E. and Garquhar, G.D. 1995. On the relationship between leaf anatomy and CO2 diffusion through the mesophyll of hypo-stomatous leaves. Plant, Cell and Environment 18: 149-157.

Tewari, R.K.; Kumar, P. and Sharma, P.N. 2010. Morphology and oxidative physiology of sulfur-deficient mulberry plants. Environmental and Experimental Botany 68: 301-308.

Thomas, S.G.; Bilsborrow, P.E.; Hocking, T.J. and Bennett, J. 2000. Effect of sulphur deficiency on the growth and metabolism of sugar beet (Beta vulgaris cv Druid). Journal of the Science of Food and Agriculture 80 : 2057 - 2062.

Varin, S.; Cliquet, J.B.; Personeni, E.; Avice, J.C. and Lemauviel-Lavenant, S. 2010. How does sulphur availability modify N acquisition of white clover (Trifolium repens L.)? Journal of Experimental Botany 61: 225-234.

Weraduwage, S.M.; Chen, J.; Anozie, F.C.; Morales, A.; Weise, E.; and Sharkey, T.D. 2015. The relationship between leaf area growth and biomass accumulation in Arabidopsis thaliana. Frontiers in Plant Science 6: 167.

Xu, H.L.; Lopez, J.; Rachidi, F.; Tremblay, N.; Gauthier, L.; Desjardins, Y, and Gosselin, A. 1996. Effect of sulphate on photosynthesis in greenhouse-grown tomato plants. Physiologia Plantarum 96: 722-726.

Zhao, F.J.; Wood, A.P. and McGrath, S.P.1999. Effects of sulphur nutrition on growth and nitrogen fixation of pea (Pisum sativum L.). Plant and Soil 212: 207-217.

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