Fine Root Biomass and Production in Tropical Moist Forest of Eastern Nepal

Tilak Prasad Gautam, Tej Narayan Mandal


The fine root biomass (< 5 mm diameter) was estimated from two soil depths (upper: 0-15 and lower: 15-30 cm) during summer, rainy and winter seasons in the undisturbed stand (US) and disturbed stand (DS) of tropical moist forest of Sunsari district in eastern Nepal. Soil samples were analyzed also for their physico-chemical characteristics. In the upper soil depth, soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP) and potassium (K) were 3.07%, 0.24%, 626.6 µg g−1, and 346.3 µg g−1 respectively in US and 1.8%, 0.14%, 618.3 µg g−1, and 268.1 µg g−1 respectively in DS. Similarly, annual mean soil microbial biomass: carbon (MB-C), nitrogen (MB-N) and phosphorus (MB-P) were 558.4 µg g−1, 50.7 µg g−1, and 12.3 µg g−1 respectively in US and 438.5 µg g−1, 39.9 µg g−1, and 9.7 µg g−1 respectively in DS. Annual mean fine root biomass (FRB), fine root production (FRP) and turnover rate was 6.6 Mg ha−1, 5.2 Mg ha−1 year−1, and 0.78 respectively in US; and 3.4 Mg ha−1, 2.9 Mg ha−1 year−1, and 0.86 respectively in DS. In comparison to upper depth, FRB decreased in lower depth by 50% in US and 55% in DS. Distinct seasonality was observed in FRB in both the stands. It was maximum in rainy and minimum in summer season. Variations in FRB due to difference in stand, season and depth were significant (P < 0.001). In US, FRB showed significant negative correlations with SOC, TP, MB-C and MB-N whereas, FRP showed significant positive correlations with them except TP. In DS, none of the soil variables showed significant correlations with FRB but SOC, TN, MB-C and MB-N showed significant negative correlations with FRP.


Forest Disturbance; Fine Root Dynamics; Soil Characteristics; Seasonal Variation; Tropical Forest.


Barbhuiya, A.; Arunachalam, A.; Pandey, H.; Khan, M. and Arunachalam, K. 2012. Fine root dynamics in undisturbed and disturbed stands of a tropical wet evergreen forest in northeast India. Tropical Ecology 53(1): 69-79.

Brookes, P.C.; Landman, A.; Pruden, G. and Jenkinson, D.S. 1985. Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biology and Biochemistry 17: 837–842.

Castellanos, J.; Jaramillo, V.J.; Sanford, R.L. and Kauffman, J.B. 2001. Slash-and-burn effects on fine root biomass and productivity in a tropical dry forest ecosystem in Mexico. Forest Ecology and Management 148: 41–50.

Comas, L.H. and Eissenstat, D.M. 2009. Patterns in root trait variation among 25 co-existing North American forest species. New Phytologist 182: 919-928.

Dipesh, K.C. and Schuler, J.L. 2013. Fine-root production and aboveground development for Loblolly pine, Silver maple and Cottonwood. Communications in Soil Science and Plant Analysis 44(14): 2207-2218.

Espeleta, J.F. and Clark, D.A. 2007. Multi-scale variation in fine-root biomass in a tropical rain forest: A seven-years study. Ecological Monographs 77(3): 377–404.

Espeleta, J.F.; West, J.B. and Donovan, L.A. 2009. Tree species fine-root demography parallels habitat specialization across a sandhill soil resource gradient. Ecology, 90: 1773–1787.

Finér, L.; Ohashi, M.; Noguchi, K. and Hirano, Y. 2011. Fine root production and turnover in forest ecosystems in relation to stand and environmental characteristics. Forest Ecology and Management 262: 2008-2023.

Gautam, T.P. and Mandal, T.N. 2013. Soil Characteristics in moist tropical forest of Sunsari District, Nepal. Nepal Journal of Science and Technology 14(1): 35-40.

Gill, R.A. and Jackson, R.B. 2000. Global patterns of root turnover for terrestrial ecosystems. New Phytologist 147: 13–31.

Girardin, C.A.J.; Aragão, L.E.O.C.; Malhi, Y.; Huaraca Huasco, W.; Metcalfe, D.B.; Durand, L.; Mamani, M.; Silva-Espejo, J.E. and Whittaker, R.J. 2013. Fine root dynamics along an elevational gradient in tropical Amazonian and Andean forests. Global Biogeochemical Cycles 27(1): 252-264.

Graefe, S.; Hertel, D. and Leuschner, C. 2010. N, P and K limitation of fine root growth along an elevation transect in tropical mountain forests. Acta Oecologica 36(6): 537-542.

Green, J.J.; Dawson, L.A.; Proctor, J.; Duff, E.I. and Elston, D.A. 2005. Fine root dynamics in a tropical rain forest is influenced by rainfall. Plant and Soil 276: 23–32.

Harteveld, M.; Hertel, D.; Wiens, M. and Leuschner, C. 2007. Spatial and temporal variability of fine root abundance and growth in tropical moist forests and agroforestry systems (Sulawesi, Indonesia). Ecotropica 13: 111–120.

Helmisaari, H.L. 2007. Roots and Carbon Allocation- Quantity, Quality and Controls. Presentation at Rootrap Session: Belowground Carbon Turnover in European Forests. Finish Forest Research Institute, Helsinki.

Hendrick, J.J.; Hendrick, R.L.; Wilson, C.A.; Michell, R.J.; Pecot, S.D. and Guo, D. 2006. Assessing the patterns and controls of fine root dynamics: An empirical test and methodological review. Journal of Ecology 94: 40-57.

Hertel, D.; Hartveld. M.A. and Leuschner, C. 2009. Conversion of a tropical forest into agroforest alters the fine root-related carbon flux to the soil. Soil Biology and Biochemistry 41: 481-490.

Hoffmann, C.W. and Usoltsev, V.A. 2001. Modelling root biomass distribution in Pinus sylvestris forests of the Turgai depression of Kazakhstan. Forest Ecology and Management 149: 103-114.

Ibrahima, A.; Mvondo, Z.E.A. and Ntonga, J. 2010. Fine root production and distribution in the tropical rainforests of south-western Cameroon: effects of soil type and selective logging. iForest - Biogeosciences and Forestry 3(5): 130-136.

Jackson, M.L. 1958. Soil Chemical Analysis. Prentice Hall, Engle-wood Cliffs, New Jersey. 498 pages.

Jimenez, E.M.; Moreno, F.H.; Penuela, M.C.; Patino, S. and Lloyd, J. 2009. Fine root dynamics for forests on contrasting soils in the Colombian Amazon. Biogeosciences 6: 2809–2827.

Jordan, C.F. 1991. Nutrient cycling processes and tropical forest management. pages 90-106, In: Gomez-Pompa, A.; Whitmore, T.C. and Hadley, M. (Editors.) Rainforest Regeneration and Management. UNESCO, Paris, France,

Joslin, J.D.; Gaudinski,J.B.; Torn, M.S.; Riley, W.J. and Hanson, P.J. 2006. Fine-root turnover patterns and their relationship to root diameter and soil depth in a C14-labeled hardwood forest. New Phytologist 172: 523–535.

Kalembasa, S.J. and Jenkinson, D.S. 1973. A comparative study of titremetric and gravimetric methods for the determination of organic carbon in soil. Journal of Science, Food and Agriculture 24: 1085–1090.

Kiley, D.K. and Schneider, R.L. 2005. Riparian roots through time, space and disturbance. Plant and Soil 269(1-2): 259-272.

Kochsiek, A.; Tan, S.and Russo, S.E. 2013. Fine root dynamics in relation to nutrients in oligotrophic Bornean rain forest soils. Plant Ecology 214: 869–882.

Kuruppuarachchi, K.A.J.M.; Seneviratne, G. and Madurapperuma, B.D. 2013. Drought induced fine root growth and canopy green-up of tropical dry zone vegetation in Sri Lanka. Journal of Tropical Forestry and Environment 3(1): 17-23.

Lee , E.H.; Tingey, D.T.; Beedlow, P.A.; Johnson, M.G. and Burdick, C.A. 2007. Relating fine root biomass to soil and climate conditions in the Pacific Northwest. Forest Ecology and Management 242: 195–208.

Lei, P.; Scherer-Lorenzen, M. and Bauhus, J. 2012. The effect of tree species diversity on fine-root production in a young temperate forest. Oecologia 169: 1105–1115.

Leuschner, C.; Harteveld, M. and Hertel, D. 2009. Consequences of increasing forest use intensity for biomass, morphology and growth of fine roots in a tropical moist forest on Sulawesi, Indonesia. Agriculture, Ecosystems and Environment 129(4): 474-481.

Lima, T.T.S.; Miranda, I.S. and Vasconcelos, S.S. 2010. Effects of water and nutrient availability on fine root growth in eastern Amazonian forest regrowth, Brazil. New Phytologist 187: 622–630.

Mandal, T.N. 1999. Ecological Analysis of Recovery of Landslide Damaged Sal Forest Ecosystem in Nepal Himalaya. Ph.D. Thesis. Banaras Hindu University, Varanasi, India. 197 pages.

Matamala, R.; Gonzalez-Meler, M.A.; Jastrow, J.D.; Norby, R.J. and Schlesinger, W.H. 2003. Impacts of fine root turnover on forest NPP and soil C sequestration potential. Science 302: 1385-1387.

Maycoc, C.R., and Congdon, R.A. 2000. Fine Root biomass and soil N and P in north Queensland rain forests. Biotropica 32(1): 185-190.

McGroddy, M., and Silver, W.L. 2000. Variations in Belowground carbon storage and soil CO2 flux rates along a wet tropical climate gradient. Biotropica 32(4): 614-624.

Meier, I.C. and Leuschner, C. 2008. Belowground drought response of European beech: fine root biomass and carbon partitioning in 14 mature stands across a precipitation gradient. Global Change Biology 14(9): 2081-2095.

Meinen, C.; Hertel, D. and Leuschner, C. 2009. Biomass and morphology of fine roots in temperate broad-leaved forests differing in tree species diversity: is there evidence of below-ground overyielding? Oecologia 161(1): 99-111.

Nadelhoffer, K.J. 2000. The potential effects of nitrogen deposition on fine-root production in forest ecosystems. New Phytologist 147: 131–139.

Noguchi, H.; Suwa,R.; de Souza, C.A.S.; da Silva, R.P.; dos Santos, J.; Higuchi, N.; Kajimoto, T. and Ishizuka, M. 2014. Examination of vertical distribution of fine root biomass in a tropical moist forest of the Central Amazon, Brazil. Japan Agricultural Research Quarterly 48(2): 231- 235.

Noordwijik, M. van; Lawson, G.; Soumare, A.; Groot, J.J.R. and Hairiah, K. 1996. Root distribution of trees and crops: Competition and/or complementarity. pages 319-364, In: Ong, C.K. and Huxley, P. (Editors) Tree-Crop Interactions: A Physiological Approach. CAB International, Wallingford, U.K.

Pei, Z.-Q.; Xiao, C.-W.; Dongm D. and Zhang, S.-R. 2012. Comparison of the fine root dynamics of Populus euphratica forests in different habitats in the lower reaches of the Tarim River in Xinjiang, China, during the growing season. Journal of Forest Research 17(4):, 343-351.

Piper, C.S. 1966. Soil and Plant Analysis. Hans Publisher, Bombay. 368 pages.

Powers, J.S. and Peréz-Aviles, D. 2013. Edaphic factors are a more important control on surface fine roots than stand age in secondary tropical dry forests. Biotropica 45(1): 1-9.

Pregitzer, K.S.; Laskowski, M.J.; Burton, A.J.; Lessard, V.C. and Zak, D.R. 1998. Variation in sugar maple root respiration with root diameter and soil depth. Tree Physiology 18: 665–670.

Richter, A.K.; Hajdas, I.; Frossard, E. and Brunner, I. 2012. Soil acidity affects fine root turnover of European beech. Plant Biosystems 147(1): 50-59.

Rieger, I.; Lang, F.; Kleinschmit, B.; Kowarik, I. and Cierjacks, A. 2013. Fine root and aboveground carbon stocks in riparian forests: the roles of diking and environmental gradients. Plant and Soil. doi: 10.1007/s11104-013-1638-8

Roderstein, M.; Hertel, D. and Leuschner, C. 2005. Above and below-ground litter production in three tropical montane forests in southern Ecuador. Journal of Tropical Ecology 21: 483-492.

Srivastava, S.K.; K.P. Singh and R.S. Upadhyay. 1986. Fine root growth dynamics in teak (Tectona grandis Linn. F). Canadian Journal of Forest Research 16: 1360–1364.

Uselman, S.M.; Qualls, R.G. and Lilienfein, J. 2007. Fine root production across a primary successional ecosystem chrono-sequence at Mt. Shasta, California. Ecosystems 10(5): 703-717.

Valverde-Barrantes, O.J.; Raich, J.W. and Russell, A.E. 2007. Fine-root mass, growth and nitrogen content for six tropical tree species. Plant and Soil 290: 357–370.

Vance, E.D.; Brookes, P.C. and Jenkinson, D.S. 1987. Microbial biomass measurements in forest soils: The use of the chloroform fumigation-incubation method in strongly acid soils. Soil Biology and Biochemistry 19: 697-702.

Vogt, K.A.; Edmonds, R.L. and Grier, C.C. 1981. Seasonal changes in biomass and vertical distribution of mycorrhizal and fibrous-textured conifer fine roots in 23- and 180-year-old subalpine Abies amabilis stands. Canadian Journal of Forest Research 11: 223–229.

Wright, S.J.; Yavitt, J.B.; Wurzburger, N.; Turner, B.L.; Tanner, E.V.J.; Sayer, E.J.; Santiago, L.S.; Kaspari, M.; Hedin, L.O.; Harms, K.E.; Garcia, M.N. and Corre, M.D. 2011. Potassium, phosphorus, or nitrogen limit root allocation, tree growth, or litter production in a lowland tropical forest. Ecology 92(8): 1616–1625.

Xiao, C.W.; Sang, W.G. and Wang, R. 2008. Fine root dynamics and turnover rate in an Asia white birch forest of Donglingshan Mountain, China. Forest Ecology and Management 255: 765–773.

Yavitt, J.B.; Harms, K.E.; Wright, S.J.; Garcia, M.N. and Mirabello, M.J. 2011. Soil fertility and fine root dynamics in response to 4 years of nutrient (N, P, K) fertilization in a lowland tropical moist forest, Panama. Austral Ecology 36: 433–445.

Yuan, Z.Y. and Chen, H.Y.H. 2010. Fine Root biomass, production, turnover rates, and nutrient contents in boreal forest ecosystems in relation to species, climate, fertility, and stand age: Literature review and meta-analyses. Critical Reviews in Plant Sciences 29(4): 204-221.

Yuan, Z.Y. and Chen, H.Y.H. 2012. A global analysis of fine root production as affected by soil nitrogen and phosphorus. Proceedings of the Royal Society B: Biological Sciences 279(1743): 3796-3802.

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