Herbivory and Chemical Defense: How is Miconia cabucu Hoehne (Melastomataceae) Reacting on a Remnant of Atlantic Rainforest?

Gabriela Thomaz da Silva, Alan Da Silva Felisbino, Patricia De Aguiar Amaral, Birgit Harter Marques

Abstract


This study examined herbivory rates and chemical defense  mechanism in leaves of Miconia cabucu Hoene  (Melastomataceae) at different development stages and environments. We also identified variations of these data in relation to seasonality and climatic factors. Young and mature leaves from 40 individuals were sampled at the border and the interior of a remnant area for the herbivory analysis. The chemical analysis was accomplished in order to detect secondary metabolites also in young and mature leaves of different development stages and environments. Young individuals were generally more attacked than adults. Higher herbivory rates were observed for mature leaves of young individuals, for both edge and interior. The chemical analysis presented positive results for phenolic compounds, flavonoids, tannins, and coumarins. Mature leaves showed higher phenolic compounds and flavonoids concentration. For the border area,higher contents was detected on summer, and for the interior, higher contents on winter. The relative air humidity and pluviosity were positively correlated with herbivory rates, while insolation and photoperiod were negatively correlated. The results of herbivory and their secondary metabolites demonstrate the importance of these relationships in Miconia cabucu, and its value for preservation and biodiversity.

Keywords


Insect-Plant Interaction; Herbivory Rates; Secondary Metabolites; Fragmentation; Seasonality.

References


AOAC. 2010. Official Methods of Analysis. 18th edition. Association of Official Analytical Chemists. .

Barbosa, V.S.; Leal, I.R.; Iannuzzi, L. and Almeida-Cortez, J. 2005. Distribution pattern of herbivorous insects in a remnant of Brazilian Atlantic forest. Neotropical Entomology 34(5): 701-711.

Bernays, E.; Lupi, A.; Bettolo, R.M.; Mastrofrancesco, C. and Tagliatesta, P. 1984. Antifeedant nature of the quinoneprimin and its quinolmiconidin from Miconia spp. Experientia 40: 1010-1011.

Brenes-Arguedes, T.; Horton, M.W.; Coley, P.D.; Lokvam, J.; Waddell, R.; Ameizoso-O’Meara, B.E. and Kursar, T.A. 2006. Contrasting mechanisms of secondary metabolite accumulation during leaf development in two tropical tree species with different leaf expansion strategies. Oecologia 149: 91-100.

Carvalho, P.E.R. 2008. Espécies arbóreas brasileiras, Volume 3. EMBRAPA, Brasilia.

Chang, C.; Yang, M.; Wen, H. and Chern, J. 2002. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 3:178-182.

Chen, M.S. 2008. Inducible direct plant defense against insect herbivores: A review. Insect Science 2:101-114.

Coley, P.D.; Bryant, J.P. and Chapin, S.F. 1985. Resource availability and plant antiherbivory defense. Science 4728: 895-899.

Cornelissen, T.G. and Fernandes, G.W. 2001. Defence, growth and nutrient allocation in the tropical shrub Bauhinia brevipes (Leguminosae). Austral Ecology 26: 246-253.

Corrêa, P.G. 2007. Defesas foliares em resposta à herbivoria em espécies lenhosas de restinga, Ipojuca, PE. MSc dissertation, Universidade Federal Rural de Pernambuco, Recife.

Costa, R.B. 2003. Fragmentação florestal e alternativas de desenvolvimento rural na Região Centro-Oeste. Pages 139 - 160. UCBD, Campo Grande.

Cunha, W.R.; Martins, C.; Silva Ferreira, D.; Crotti, A.E.; Lopes, N.P. and Albuquerque, S. 2003. In vitro trypanocidal activity of triterpenes from Miconia species. Planta Medica 69: 470-472.

Cunha, W.R.; Crevelin, E.J.; Arantes, G.M.; Crotti, A.E.M.; Andrade e Silva, M.L.; Cardoso Furtado, N.A.J.; Albuquerque, S. and Silva Ferreira, D. 2006. A study of the trypanocidal activity of triterpene acids isolated from Miconia species. Phytotherapy Research 6: 474-478.

Del-Claro, K. and Torezan-Silingardi, H.M. 2012. Ecologia das Interações Plantas-Animais: uma Abordagem Ecológico-Evolutiva. 1st Edition, Technical Books, Rio de Janeiro. 336 pages.

Dirzo, R. and Dominguez, C. 1995. Plant-herbivore interactions in Mesoamerican tropical dry forest. Pages 304-325. In: Bullock, S.H.; Mooney, H.A. and Medina, E.A. (Editors) Seasonally Dry Tropical Forests. Cambridge University Press, Cambridge.

Eyles, A.; Bonello, P.; Ganley, R. and Mohammed, C. 2010.Induced resistance to pests and pathogens in trees. New Phytologist 4: 865-1119.

Gurevitch, J.; Scheiner, S.M. and Fox, G.A. 2009. Ecologia vegetal. 2nd Edition. Artmed, Porto Alegre. 592 pages.

Hammer, O.; Harper, D.A.T. and Ryan, P.D. 2001.PAST: Paleontological statistics software package for education and data analyses. Paleontological Electronica 4(1): 1-9.

Herrera, C.M. and Pellmyr, O. 2002. Plant-Animal Interactions: An Evolutionary Approach. Blackwell Science, Oxford, UK. 313 pages.

Kogan, M. and Paxton, J. 1983.Natural Inducers of Plant Resistance to Insects. Pages 153-171, In: Hedin, P.A. (Editor) Plant Resistance to Insects. ACS Symposium Series, volume 208. American Chemical Society, Washington, DC.

Köppen, W. 1931. Grundriss der Klimakunde. Gruyter, Berlin. 369 pages.

Kruidhof, H.M.; Allison, J.D. and Hare, J.D. 2012.Abiotic induction affects the costs and benefits of inducible herbivore defenses in Datura wrigthii. Journal of Chemical Ecology 10:1215-1224.

Liimatainen, J.; Karonen, M.; Sinkkonen, J.; Helander, M. and Salminen, J. 2012. Phenolic compounds of the inner bark of Betula pendula: Seasonal and genetic variation and induction by wounding. Journal of Chemical Ecology 11: 1410-1418.

Luengas-Caicedoa, P.E.; Braga, F.C.; Brandão, G.C. and Oliveira, A.B. 2007. Seasonal and intraspecific variation of flavonoids and proanthocyanidins in Cecropia glaziovi Sneth. leaves from native and cultivated specimens. Zeitschrift für Naturforschung 62c: 701-709.

Maron, J.L. and Gardner, S.N. 2000. Consumer pressure, seed versus safe-site limitation, and plant population dynamics. Oecologia 124: 260-269.

Marquis, R.J. 1984. Leaf herbivores decrease fitness of a tropical plant. Science 4674: 537-539.

Medeiros, J.D. and Morretes, B.L. 1995. Dimensões da Folha e Herbivoria em Miconia cabucu Hoehne (Melastomataceae). Biotemas. 1: 97-112.

Medinaceli, A.; Miranda-Avilés, F.; Flores-Saldaña, N.P. and Gutierrez-Calucho, E. 2004. Herbivoría en relación altamaño de la planta y a lãs diferencias de exposición de Pilea sp. (Urticaceae) enlaEstación Biológica Tunquini, Cotapata, La Paz, Bolivia. Ecología en Bolivia 2: 4-8.

Murcia, C. 1995. Edge effects in fragmented forests: implications for conservation. Tree 2: 58-62.

Norusis, M.J. 2008. SPSS 17.0 Guide to Data Analysis. Prentice -Hall, New Jersey. 672 pages.

Peixoto-Sobrinho, T.J.S.; Cardoso, K.C.M.; Gomes, T.L.B.; Albuquerque, U.P.and Amorim, E.L.C. 2009. Análise da pluviosidade e do efeito de borda sobre os teores de flavonóides em Bauhiniacheilantha (Bong.) Steud. Fabaceae. Revista Brasileira de Farmacognosia. 3: 740-745.

Plano de Manejo do Parque Estadual da Serra Furada. 2009. Volume 1: Diagnóstico e Planejamento / Fundação do Meio Ambiente P712 PPMA/SC. Socioambiental Consultores Associados Ltda.

Pourmorad, F.; Hosseinimehr, S.J. and Shahabimajd, N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnolgy 11: 1142-1145.

Ribeiro, M.T.; Ramos, F.N.and Santos, F.A.M. 2009. Tree structure and richness in an Atlantic forest fragment: distance from anthropogenic and natural edges. Revista Árvore 6: 1123-1132.

Rocha, C.F.D.; Bergallo, H.D.; Van Sluys, M. and Alves, M.A.S. 2006. Biologia da conservação: essências. RiMA, São Carlos. 582 pages.

Rodrigues, J. 2007. Uso sustentável da biodiversidade brasileira: prospecção químico-farmacológica em plantas superiores: Miconia sp. Msc dissertation. Universidade Estadual Paulista, Araraquara.

Santos, G.;Ayub, A.; Sarmento,R.and Almeida-Cortez, J. 2007. Herbivoria foliar em plântulas e adultos de Cordia nodosa Lam. (Boraginaceae) em um remanescente de mata atlântica, Pernambuco.In: Congresso de Ecologia do Brasil, SEB Anais, Caxambu, São Paulo.

Schoonhoven, L.M.; Jermy, T. and van Loon, J.J.A. 1998. Insect-Plant Biology: From Physiology to Evolution. Chapman & Hall, London. 409 pages.

Serpeloni, J.M.; Reis, M.B.; Rodrigues, J.; Santos, L.C.; Vilegas, W.; Varanda, E.A.; Dokkedal, A.L. and Cólus, I.M.S. 2008. In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test. Mutagenesis 6: 501-507.

Sevegnani, L. 2007. A herbivoria como limitador do desenvolvi-mento e sobre vivência das plantas na floresta. Pages 25-36, In: Rego, G.M.; Negrelle, R.R.B and Morellato, L.P.C. Fenologia: ferramenta para conservação, melhoramento e manejo de recursos vegetais arbóreos. Embrapa Florestas, Colombo, PR.

Singleton, V.L. and Rossi, J.A. JR. 1965. Colorimetry of total phenolics with phosphomolybdic - phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144-158.

Sistema Meteorológico do Paraná –SIMEPAR. 2017. Centro Politécnico da UFPR, Curitiba, Paraná, Brasil.

Wagner, H. and Bladt, S. 2009. Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd Edition. Springer Science & Business Media, Berlin. 384 pages

Yariwake, J.H.; Lanças, F.M.; Cappelaro, E.A.; Vasconcelos, E.C.; Tiberti, L.A.; Pereira, A.M.S. and Franca, S.C. 2005. Variabilidade sazonal de constituintes químicos (triterpenos, flavonóides e polifenóis) das folhas de Maytenus aquifolium Mart. (Celastraceae).Revista Brasileira de Farmacognosia. 2: 162-168.

Zvereva, E.L. and Kozlov, M.V. 2001. Effects of pollution-induced habitat disturbance on the response of willows to simulated herbivory. Journal of Ecology 89: 21-30.


Full Text: PDF

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

COPYRIGHT of this Journal vests fully with the National Instional Institute of Ecology. Any commercial use of the content on this site in any form is legally prohibited.